Alongside analytical services we also provide training courses on Shimadzu Nexera XP HPLC and Shimadzu LCMS-8040, these include:

  • 3 day HPLC analysis
  • 5 day LCMS analysis
  • 1 day HPLC refresher course
  • 1 day general laboratory skills

To get an understanding of the benefits of our courses, we asked those who had been previously trained questions about their experience here. Some answers where extracted from previously submits surveys, shown with Anonymous (Anon).

  1. Which training course did you follow?

E: I came in on a summer internship to gain some relevant experience for my degree and was trained up to run all the instruments in the lab.

M: LC-MS 5 day course.

  1. Why did you choose the Bio Analysis Centre for training?

E: It is a small team so I knew I would get good 1 on 1 training in a quiet setting where I could learn.

M: Course content and excellent reviews.

  1. How did the training received compare to what knowledge you previously had?

E: I had very little previous knowledge, maybe some basics of the principles behind the techniques but no idea how to run the machines or how to set up a practical on them.
In that sense I was taught everything from scratch, even my basic lab skills where greatly improved during this training.

M: It was an intensive 5-day course that covered all aspects of MS thoroughly and in great depth.

  1. What did you hope to gain from your training course?

E: Some new skills to be able to add to my CV, they are such unusual skills to have for someone my age and I was very grateful for the opportunity to learn new things.

M: My aim was to gain hands-on experience in Mass Spectrometry. I wanted to be able to run analysis independently.

  1. Was there anything else you would’ve liked to have learnt?

E: No Comment

M: The course covered everything I needed.

Anon: More on the Mass Spectrometer if had more time and more method development on LC + LCMS

Anon 2: General lab practice such as pipettes and weights calibration

  1. Were there any areas that could’ve been improved?

E: No not that I can think of.

M: No comment.

Anon: Some basic theory at the beginning of the course to cater for beginners, however it was addressed as we went along

  1. Would you recommend our training programmes to a colleague?

E: Definitely, if anyone said they need this sort of work doing I would encourage them to come learn how to do it themselves, it’s a great thing to know how to do.

M: I would highly recommend this course to colleagues and other PhD students who would want to gain experience in Mass Spectrometry.

For the Following statements:

Q8 Services Provided,
Q9 Quality of training,
Q10 Interaction with trainer,
Q11 Understanding of the topic,

we asked the participants in the survey to rate on a scale from 1 – 5, 1 being very bad and 5 very good, how their training was. All results, which can be seen in Graph 1 below, came back with very good (5) as the answer to all.

Graph 1


Graph 2


Graph 2 above shows previously gathered information along side the new data of the participant’s overall enjoyment of training at the BAC, with an average of 4.5 / 5 between 10 participants.

Alongside the overall enjoyment these comments where left:

Great training course in a great facility, enjoyed my time being trained there by Cali and Amy. Thank you!

Thanks to Cali and the team for a great training course. It has been really informative and useful for our research, Cali’s expertise allowed us to tailor the training to our specific need.

Our experience with Cali at the Bio Analysis Centre was really constructive, she provided us with a lot of insight and tips for our type of experiment. Highly recommended.

Dr Carolyn Hyde is very experienced teaching expert on this programme and a great manager too! We will talk more on all possible collaborations. Many thanks for sharing all the information and knowledge!


Here at the Bio Analysis Centre we have the capability to run samples through our UHPLC (Ultra High Performance Liquid Chromatograph) or LCMS (Liquid Chromatograph Mass Spectrometer). Shimadzu (1), the manufacture of the machinery, ran a Multiple Residue Analysis of 210 Pesticides in Food Samples by Triple Quadrupole UHPLC – MS/MS.


Pesticide residue on food can severely affect human health, so governments and food manufacturers established regulations which set the limits in a range of 0.01 – 10 mg / kg depending on the toxicity of the pesticide. For food used in the production of baby foods, the detectable level of pesticide must be less than 0.01 mg / kg along with prohibiting the use of certain toxic pesticides.


Linearity was investigated over a nine point calibration, with samples ranging from 0.5 μg / kg – 0.2mg / kg or 0.5 – 200 parts per billion (ppb) analysed in duplicate; calibration samples were injected once in increasing order and once in decreasing order. From that the Mass Spectrometer calculated from the area of the peak the concentration by correlating to the calibration curve associated with the specific pesticide.


This exact assay can be conducted here at the Bio Analysis Centre, if you have a similar project or any enquiries for any other assay, please contact us.



  1. Multi-Residue Analysis of 210 Pesticides in Food Samples by Triple Quadrupole UHPLC-MS/MS [Internet]. Shimadzu; [cited 29 January 2020]. Available from: https://www.shimadzu.com/an/literature/lcms/jpo113017.html?fbclid=IwAR0vWv-SXKE4BKlRmm_RgKE7JkAFXR9i-JJn3KsZRGb2sYxMwxiyaWKkNWk
Bio-Analysis at the One Nucleus Bio Wednesday 2019

Bio-Wednesday One Nucleus hosted by LBIC at the RVC

We were delighted to be asked to join the panel last night at the One Nucleus event hosted by LBIC at the RVC.


The topic, ‘Staffing the 21st Century Life Sciences R&D Industry.’ bio-analysis HPLC and LCMS

As an employer of several graduates, undergraduates and summer placements in our 5 years of business, we were interested to be part of the discussion and hear other people’s point of view.

We have found the quality of students coming through to be excellent and a real asset to us. In a business like ours they receive a very hands on and varied approach to the commercial world of bio-science.  (A hugely different experience from starting out in a Big Pharma Organisation!)

Discussion topics resonated around what the future looks like for graduates and post graduates; what skills employers and recruitment professionals are looking for: risk takers, lateral thinkers, commercial acumen; what could be taught, what should be taught and the changing foot print of career paths.

For a business like ours, people skills are essential, along with self motivation and common sense.


We pride ourselves in offering an excellent technical and customer service.  Our HPLC and LC-MS training can be combined with method development and bespoke consultancy to help advance your Research and Development.  Once trained, clients can come back and use the equipment to continue their research projects.

As a business and an employer, we have a lot to offer so please get in touch.


Bio-Analysis at the On Nucleus Bio-Weds - HPLC and LCMS




Atypical Myopathy Testing Now Available (In Conjunction With the Royal Veterinary College)


The Royal Veterinary College (RVC) and the Bio-Analysis Centre are now conducting testing for atypical myopathy as part of the RVC’s work towards improved treatments and management of this disorder, and to enhance the welfare of affected horses. Atypical myopathy of horses is a severe and life threatening equine muscle disorder that is caused by the ingestion of Sycamore tree seeds, leaves or seedlings by horses that are kept at pasture. Risk factors for horses remain unclear. It is, for example, not currently known whether some trees are more toxic than others or whether the amount of toxin varies at certain times of the year or with certain climatic conditions. The RVC is working to help horse owners to gain a better understanding of the condition.

Following research that was supported by The Horse Trust and the RVC’s Animal Care Trust (ACT), the Comparative Neuromuscular Diseases Laboratory at the RVC is now offering testing of seeds, seedlings and leaves for the hypoglycin A toxin known to cause this disorder. To find out if plant samples from your property contain the toxin known to cause atypical myopathy, you can now send samples directly to the lab where they will be tested at a subsidised cost of £50.   In addition, the Comparative Neuromuscular Diseases Laboratory is also offering testing of horse blood and urine samples, submitted by your vet, if they suspect atypical myopathy or in field companions. This should help to establish a much more rapid and accurate diagnosis, and subsequent treatment, than with previous tests.

Professor Richard Piercy, Professor of Comparative Neuromuscular Disease, said: “We’re really pleased to be able to launch our testing service for owners who may be concerned about their horses. With the support of the Horse Trust and ACT, and through working with owners in this way, we hope to be able to improve the understanding of atypical myopathy and improve the welfare of horses with this severe condition.”

Full details, including prices and packaging instructions are available here.



2018 Update: The work carried out by Carolyn and Imogen in developing this assay has recently been published in Plos One, along with two others from the Neuromuscular Research Group at the RVC. You can read it here.



bio-analysis Centre

Bio-Analysis Centre Now Open for Business

Welcome to the Bio-Analysis Centre based in the London Bioscience Innovation Centre (LBIC).

Our Bio-Analysis Centre has over 20 years experience in LC-MS and HPLC analysis.



We use the Shimadzu Nexera XR HPLC system. These instruments have cooled autosamplers (for sample integrity) and Photo-Diode Array detectors, which can be used for compound analysis from small drug-like molecules to proteins. This instrument provides the optimal solution for a diverse range of applications. Due to the modular design of these systems, other detectors can be added as required, for example Evaporative Light Scattering (ELS), Refractive Index (RI) or Fluorescence. More pump modules could also be added if required.

Mass Spectrometry

Mass Spectrometry

We use a triple quadrupole mass spectrometer (Shimadzu LCMS-8040) with highly sensitivity, reliable and fast results. We have extensive experience and knowledge in method development for new compounds along with excellent expertise in interpretation of results – providing a complete service to your analytical requirements. If required, another detection mode can be added, for example UV, Fluorescence or Refractive Index (RI) to complement the mass data.



We use one of deltaDOT’s latest instruments (HPCE-512) which separates and analyses a wide range of samples - from small molecules and amino acids, through to proteins and virus-like particles. It uses an advanced form of label free Capillary Electrophoresis which produces faster, higher quality results at a fraction of the cost of many other approaches.