AlphaLink is a new workflow which uses AlphaFold2 with XL-MS for better protein structure predictions

10 min read          AlphaFold Protein structures are defined, experimentally, by X-ray crystallography, nuclear magnetic resonance and electron cryomicroscopy (cryo-EM). However, these techniques are complex, time consuming, expensive and the protein is often not in its native form (Bertoline et al., 2023). Therefore, from its introduction in 2018, DeepMind’s AlphaFold program has […]

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Adeno-associated virus (AAV) vector manufacturing for gene therapy products: How does the LCMS and HPLC support good manufacturing practice?

20 min read. The adeno-associated virus (AAV) was first discovered in 1965 as a contaminant of adenovirus preparations, subsequently AAV was found in human tissues. Pure scientific curiosity in understanding basic AAV biology is what led to realising the tremendous potential of AAV as a vector in human gene therapies. AAV vectors are now the […]

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DeltaDOT GFR test – Should this be the standard kidney function test in paediatric and advanced staged cancer patients?

15 min read. Chemotherapeutic agents used to treat cancer require accurate dosing to prevent serious adverse toxicities. For those drugs excreted through the kidney, a precise understanding of kidney function (as measured by glomerular filtration rate, GFR) is needed to ensure drug toxicity is avoided. Carboplatin is unique among chemotherapeutic agents in that its dosing […]

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Detection of Breast Cancer‐Specific Extracellular Vesicles with Fiber‐Optic SPR Biosensor

Extracellular vesicles (EVs) play a crucial role in mediating cell-to-cell communication. They are involved in several physiological and pathological processes within the body, including cancer development, and potential use as non-invasive cancer biomarkers. However, the extensive heterogeneity in size, composition, and origin of EVs makes their accurate and reliable characterization and detection crucial to meet […]

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Domestic Animal Endocrinology

Publishing a research paper is a significant milestone in any academic or professional career. It’s an achievement that requires hard work and dedication. We wanted to take a moment to acknowledge our lab manager’s Dr. Carolyn Hyde, amazing work on this recent research paper, “Domestic Animal Endocrinology”.  Let’s take a summarised dive into this blog […]

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bioanalysis

LC-MS in testing of drugs of abuse

According to national statistics published by the government, between April 2020 and March 2021, 275,896 adults came into contact with drug and alcohol services (1). Testing for drugs of abuse can be  used to a) discover drug abuse; b) monitor someone who has a substance abuse problem; c) detect drug intoxication and d) determine the […]

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WHAT IT’S LIKED TO BE TRAINED AT THE BIO ANALYSIS CENTRE.

Alongside analytical services we also provide training courses on Shimadzu Nexera XP HPLC and Shimadzu LCMS-8040, these include: 3 day HPLC analysis 5 day LCMS analysis 1 day HPLC refresher course 1 day general laboratory skills To get an understanding of the benefits of our courses, we asked those who had been previously trained questions […]

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WHAT CAN WE DO FOR YOU?

Here at the Bio Analysis Centre we have the capability to run samples through our UHPLC (Ultra High Performance Liquid Chromatograph) or LCMS (Liquid Chromatograph Mass Spectrometer). Shimadzu (1), the manufacture of the machinery, ran a Multiple Residue Analysis of 210 Pesticides in Food Samples by Triple Quadrupole UHPLC – MS/MS.   Pesticide residue on […]

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