HPLC Services

hplc high performance liquid chromatography

Our HPLC services use the Shimadzu Nexera XR system.  These analytical instruments have cooled autosamplers (for sample integrity) and Photo-Diode Array detectors, which can be used for compound analysis, from small drug-like molecules to proteins.

 

If you have a need for small-scale purifications we also have a fraction collector.

Other forms of detection listed below are available upon request:

  • Fluorescence
  • Refractive Index
  • Conductivity
  • Evaporative Light Scattering

When sourcing our analysis equipment we have ensured that accuracy and reproducibility of solvent delivery and injection volume were key performance aspects. Shimadzu addresses these features by incorporating superb technology as a fundamental element.  Furthermore, improved hardware components support high-speed analyses, which have accelerated via advances in column technology.

 

hplc high performance liquid chromatography serviceFlexibility – Due to its modular design, the Nexera XR allows configuration of systems geared to meet specific application requirements. Each module delivers an excellent, reliable performance across an extensive analytical range.

 

Method Compatibility – Constructed with a focus on compatibility with general HPLC systems, the Nexera XR is optimised for method transfer from competing systems. Additionally, the system capacity can be changed to suit the objective, enable smooth HPLC method transfers.

 

Ultra-Sensitive Detectors – The SPD-M20A photodiode array offers extremely advanced levels of sensitivity. It has a high-sensitivity mode with an 8nm slit width. By using light-source compensation, it offers a noise level of 0.6×10-5AU, comparable to that of traditional UV-VIS detectors. Improving the linearity provides clients with a wide dynamic range and allows for the analysis of a target compound and minor impurities in a single run. Being equipped with a temperature control function to suppress the effects of room temperature fluctuations allows these detectors to achieve excellent baseline stability and analysis reliability.

Refractive Index Detector (RID)

The Bio-Analysis Centre has recently acquired a Refractive Index Detector to add to our detection capabilities. The detection principle involves measuring the change in refractive index (RI) of the column effluent passing through the flow cell. The flow cell contains two parts, one for the sample and one for the reference solvent. The detector measures the RI of both components. When only solvent passes through the component, the measured RI of both components is the same, but when an analyte passes through the flow cell, the two measured refractive indexes are different, which appears as a peak in the chromatogram.

An RID is considered to be a universal detector because it can detect anything with a refractive index different from the solvent.
Please contact us using the enquiry form on the right if you would like to know more about our capabilities.

HPLC Training

In addition to providing a coherent service, the Bio-Analysis Centre offers training on the HPLC. Training usually lasts 3 days and certification is provided following completion of the course. Please see our training page for more information.

 

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