HPCE or High Performance Capillary Electrophoresis is applied in Life Sciences research, drug testing, diagnostics and bio-pharmaceutical manufacturing. It is used in detection, separation and analysis of proteins, nucleic acids, carbohydrates, viruses and bacteria, chemicals and a wide range of other analytes. deltaDOT design, manufacture and produce HPCE instruments which are marketed worldwide. We use one of deltaDOT’s latest instruments (HPCE-512) which can separate and analyse a wide range of samples from small molecules and amino acids through to proteins and Virus-Like Particles. These instruments use deltaDOT’s proprietary imaging and analysis technology called Label Free Intrinsic Imaging (LFII®). High Performance Capillary Electrophoresis and Label Free Intrinsic Imaging offers dramatically improved discovery power in comparison to other conventional CE systems.
The deltaDOT instrument differs from conventional CE as it gathers data from 512 detection points and uses proprietary signal processing algorithms. Through these methods, the technology is able to produce higher quality results than competing CE systems. The software – derived from particle physics – can determine which of the signals relate to the sample and which do not, allowing elimination of extraneous effects and inaccuracies. The machines advanced signal processing technology combined with multi-point detection produces high quality data – in terms of sensitivity, resolution, quantification and repeatability.
The data analysis of biomolecules performed using this system is enabled by proprietary Equiphase Vertexing Algorithm (EVA) and Generalised Separation Transform (GST) algorithms. GST is a method of combining the data from the multiple pixels in a natural way which preserves the peak shape information of the electropherograms while at the same time maximising the signal-to-noise ratio.
Conventional Capillary Electrophoresis systems use single point detection, meaning each band of biomolecules is measured by one detector. This often requires biomolecules to be labelled with radiochemical, fluorescent, calorimetric or luminescent compounds. The use of labels means that such CE instruments are unable to differentiate between extraneous interface, electrical noise, light source fluctuations and other undesirable effects.